SEMESTER LEARNING PLAN
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Course Title: Basic Biomolecular Engineering (TDB)
MK code: AKM21 442
Credit Weight: 2
Group of Courts: Elective
Semester: 6
Prerequisite Course: Bio1
Lecturer:
Dr. Agustina LN Aminin, M.Si,
Dr. M. Asy’ari, M.Si,
Dra. Nies Suci Mulyani, MS.,
Purbowatiningrum RS., M.Si.
Graduate Learning Outcomes (GLO)
| Attitude | GLO1-(S9) | Demonstrate an attitude of responsibility for work in their field of expertise independently. |
| Knowledge | GLO2-(PP1) | Mastering the theoretical concepts of structure, properties, changes, kinetics, and energetics of molecules and chemical systems, identification, separation, characterization, transformation, synthesis of micromolecular chemicals, and their application. |
| General Skills | GLO 3 -(KU1) | Able to apply logical, critical, systematic, and innovative thinking in the context of the development or implementation of science and technology that pays attention to and uses humanities values by their field of expertise |
| Special skill | GLO 4 -(KK3) | Able to analyze several alternative solutions in identification, analysis, isolation, transformation, and synthesis of available chemicals and present analysis conclusions for appropriate decision making. |
Course Description
The main objective of the program is for students to study research techniques in Biochemistry. This course includes a multidisciplinary approach in the study of various biological macromolecules using modern methods. These include methods for purifying proteins, revealing recombinant proteins in bacterial cells, and analyzing biological molecules by electrophoresis, Western blotting, and enzyme activity assays.
| Week | Expected ability (Sub-CLO) | Study Materials/ Learning Materials | Learning methods | Student Learning Experience | Time (minutes) | Evaluation | |
| Criteria and Indicators | % | ||||||
| 1 | Able to understand (C2), construct (P4), and discuss (A2) Cell Characteristics and Biomolecules | Characteristics of Cells and Biomolecules:
1. Properties of Prokaryotic and Eukaryotic Cells 2. Biomolecular Properties 3. Occupational Safety in Biochemical Research |
Discovery learning
Cooperative learning |
Students discuss | FF: 1 x (2 x 50”)
ST + SS: 1 x [(2 x 50”) + (2 x 60”)] |
(1). Accuracy describes the Characteristics of Cells and Biomolecules.
(2). Student activity in discussions and doing assignments. |
5 |
| 2 | Able to understand (C2), construct (P4), and discuss (A2) Media Making Techniques for Cell Growth and Cell Isolation and Screening | Microbiological Engineering:
1. Media Making Techniques for Cell Growth 2. Cell Isolation and Screening 3. Microbiological Engineering |
Discovery learning
Cooperative learning |
Students discuss, | FF: 1 x (2 x 50”)
ST + SS: 1 x [(2 x 50”) + (2 x 60”)] |
(1). Accuracy in describing Media Making Techniques for Cell Growth, Isolation, and Cell Screening.
(2). Student activity in discussions and doing assignments. |
5 |
| 3 | Able to understand (C2), construct (P4), and discuss (A2) Cell Inoculation and Cell Storage Techniques | Cell Inoculation
4. Cell Storage Techniques |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | FF: 1 x (2 x 50”)
ST + SS: 1 x [(2 x 50”) + (2 x 60”)] |
(1). Accuracy describes Cell Inoculation and Cell Storage Techniques.
(2). Student activity in discussions and doing assignments. |
5 |
| 4 | Able to understand (C2), construct (P4), and discuss (A2) Enzyme/ Protein Isolation Techniques and Enzyme/ Protein Purification Techniques | Isolation, Purification, and Characterization of Enzymes
1. Enzyme/Protein Isolation Technique 2. Enzyme/ Protein Purification Techniques |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | FF: 2 x 50
ST: 2 x 60 SS: 2 x 60 |
(1). Accuracy describes Enzyme/ Protein Isolation Techniques and Enzyme/ Protein Purification Techniques.
(2). Student activity in discussions and doing assignments. |
10 |
| 5 | Able to understand (C2), construct (P4), and discuss (A2) Enzyme/ Protein Purification Techniques (continued) and Enzyme/ Protein Characterization Techniques | Isolation, Purification, and Characterization of Enzymes
2. Enzyme/Protein Purification Techniques (continued) 3. Enzyme/ Protein Characterization Techniques |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | FF: 2 x 50
ST: 2 x 60 SS: 2 x 60 |
(1). Accuracy describes Enzyme/ Protein Purification Techniques and Enzyme/ Protein Characterization Techniques.
(2). Student activity in discussions and doing assignments |
10 |
| 6 | Able to understand (C2), construct (P4), and discuss (A2) Types of Enzyme Immobilization Techniques and Enzyme Immobilization Methods | Enzyme Immobilization
1. Types of Enzyme Immobilization Techniques 2. Enzyme Immobilization Method |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | FF: 2 x 50
ST: 2 x 60 SS: 2 x 60 |
1). Accuracy describes the types of Enzyme Immobilization Techniques and Enzyme Immobilization Methods.
(2). Student activity in discussions and doing assignments. |
10 |
| 7 | Able to understand (C2), construct (P4), and discuss (A2) SDS-PAGE and Western blot techniques | Modern Protein Techniques
1. sodium dodecyl sulfate-polyacrylamide gel electrophoresis 2. Western Blot |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | FF: 2 x 50
ST: 2 x 60 SS: 2 x 60 |
(1). Accuracy describes the SDS-PAGE and Western blot techniques
(2). Student activity in discussions and doing assignments. |
10 |
| 8 | Midterm exam | Written exam | 90 | Truth in solving exam questions | |||
| 9 | Able to understand (C2), construct (P4), and discuss (A2) Nucleic Acid Isolation and Nucleic Acid Purification Teknik | Isolation, Purification and Characterization of Nucleic Acids
1. Nucleic Acid Isolation Technique 2. Nucleic Acid Purification Technique |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | FF: 2 x 50
ST: 2 x 60 SS: 2 x 60 |
(1). Accuracy describes the Nucleic Acid Isolation and Nucleic Acid Purification Techniques
(2). Student activity in discussions and doing assignments |
10 |
| 10 | Able to understand (C2), construct (P4), and discuss (A2) Nucleic Acid Characterization Techniques | Isolation, Purification, and Characterization of Nucleic Acids
3. Nucleic Acid Characterization Technique |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | FF: 2 x 50
ST: 2 x 60 SS: 2 x 60 |
(1). Accuracy describes the technique of Nucleic Acid Characterization Techniques.
(2). Student activity in discussions and doing assignments. |
10 |
| 11 | Able to understand (C2), construct (P4), and discuss (A2) Hybridization Techniques and Immunology Techniques (ELISA, RIA) | Advanced Biomolecular Engineering
1. Hybridization Techniques (Southern Blotting, Northern Blotting, Western Blotting) 2. Immunological Techniques (ELISA, RIA) |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | FF: 2 x 50
ST: 2 x 60 SS: 2 x 60 |
(1). Accuracy in describing Hybridization Techniques and Immunology Techniques (ELISA, RIA).
(2). Student activity in discussions and doing assignments. |
10 |
| 12 | Able to understand (C2), construct (P4), and discuss (A2) Cloning Technique: Cell Transformation | Advanced Biomolecular Engineering
3. Cloning Technique: Cell Transformation |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | FF: 2 x 50
ST: 2 x 60 SS: 2 x 60 |
(1). Accurately describes Cloning Technique: Cell Transformation.
(2). Student activity in discussions and doing assignments
|
5 |
| 13 | Able to understand (C2), construct (P4), and discuss (A2) PCR and Sequencing Techniques | Advanced Biomolecular Techniques
4. PCR technique 5. Sequencing |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | FF: 2 x 50
ST: 2 x 60 SS: 2 x 60 |
(1). Accuracy describes PCR and Sequencing Techniques.
(2). Student activity in discussions and doing assignments. |
5 |
| 14 | Able to understand (C2), construct (P4), and discuss (A2) Protein crystallization technique | Biophysical Engineering
1. Protein crystallization |
Discovery learning
Cooperative learning Problem Based Learning |
Students discuss, | (1). Accuracy describes the Protein crystalization technique.
(2). Student activity in discussions and doing assignments. |
5 | |
| 15 | Able to understand (C2), construct (P4), and discuss (A2) Fluorescence spectrophotometry and Differential scanning calorimetry Techniques | Biophysical Engineering
2. Fluorescence spectrophotometry 3. Differential scanning calorimetry (DSC) for studying protein |
Discovery learning
Cooperative learning Problem Based Learning |
Students work on practice questions to discuss | (1). Accuracy describes the technique of Fluorescence spectrophotometry and Differential scanning calorimetry.
(2). Student activity in discussions and doing assignments. |
5 | |
| 16 | Final exams | Written exam | 90 | The truth and completeness of the answer to the question | |||
| Total Rating | 100 | ||||||
Reference:
- Pelczar, M.J. dan Chan, E.C.S (1986), Dasar-Dasar Mikrobiologi, UI-Press, Jakarta
- Slamet Sudarmadji (1996), Teknik Analisa Biokimiawi, Liberty, Yogyakarta
- Deutscher, M.P. (1990), Guide to Protein Purification, Academic Press Limited, London
Glossary
GLO = Graduate Learning Outcome
CLO = Course Learning Outcomes
FF = Face to Face Learning
ST = Structured tasks
SS = Self Study
