SEMESTER LEARNING PLAN
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Course Title: Experimental Biochemistry (PBiK)
MK code: AKM21 555
Credit Weight: 1
Group of Courts: Compulsory
Semester: 5
Prerequisite Course: Bio1
Lecturer:
Dr. M. Asy’ari, M.Si,
Graduate Learning Outcomes (GLO)
| Attitude | GLO1-(S9) | Demonstrate an attitude of being responsible for work in their field of expertise independently |
| Knowledge | GLO2-(PP1) | Mastering the theoretical concepts of structure, properties, changes, kinetics, and energetics of molecules and chemical systems, identification, separation, characterization, transformation, synthesis of micromolecular chemicals, and their application |
| General Skills | GLO3 -(KU1) | Able to apply logical, critical, systematic, and innovative thinking in the development or implementation of science and technology that pays attention to and applies humanities values by their field of expertise |
| Special skill | GLO4 -(KK1) | Able to produce appropriate conclusions based on the identification, analysis, isolation, transformation, and synthesis of chemicals that have been carried out |
| GLO5 -(KK2) | Able to solve science and technology problems in general chemistry and uncomplicated scopes such as identification, analysis, isolation, transformation, and synthesis of micro-molecules through the application of knowledge of structure, properties, kinetics, and energetics of molecules and chemical systems, with methods analysis and synthesis in specific chemical fields, as well as the application of relevant technologies | |
| GLO6 -(KK3) | Able to analyze several alternative solutions in identification, analysis, isolation, transformation, and synthesis of available chemicals and present analysis conclusions for appropriate decision making | |
| GLO7 -(KK4) | Able to use software to determine the structure and energy of micromolecules, software to assist analysis and synthesis in general or more specific chemical fields (organic, biochemical, or inorganic), and data processing (analytical chemistry) |
Course Description
This course practices several experiments which include: qualitative and quantitative analysis of lipids, the effect of heating and inhibitors on enzyme activity, identification of compounds in urine, microbiology: Isolation and cultivation of microbes, isolation, and purification of early stages of -amylase enzymes, determination of specific activities of -amylase enzymes. -amylase and bioinformatics: a phylogenetic analysis.
| Week | Expected ability (Sub-CLO) | Study Materials/ Learning Materials | Learning methods | Student Learning Experience | Time (minutes) | Evaluation | |
| Criteria and Indicators | % | ||||||
| 1 | Preparation of Practical Tools and Materials | ||||||
| 2 | Practical Assistant | ||||||
| 3 | Able to describe (C2) the principles and stages of lipid analysis and implement them (P2) in qualitative and quantitative analysis experiments of lipid-containing samples using laboratory experiments and chemical reaction equations with a minimum accuracy of 70% | Biomolecules, Compound Reactions, Qualitative Analysis, Qualitative Analysis
LIPID: Qualitative and Quantitative Analysis Qualitative Analysis: – Peroxide Test – Phosphate test on lecithin – Cholesterol test (Lieberman-Burchard) Quantitative Analysis: – Determination of the iodine number – Determination of the number of saponification |
Project Based Learning | Students learn by doing tasks (lipid experiments) that have been systematically designed.
Students learn to show performance and account for their work to the assistant every week and present it in a discussion forum at the end of the practicum. |
FF: 1 x (3 x 60 min); ST:
1 x (3 x 60 min); SS: 1 x (3 x 60 min) |
(1). accuracy describes the principles and steps of the method of lipid analysis.
(2). The accuracy of applying the method in the qualitative and quantitative analysis of samples of lipid-containing materials. (3). Student activity in practicum and doing assignments (reports and presentations). |
10 |
| 4 | Able to describe (C2) the effect of heating and inhibitor factors on protein properties and implement it (P2) in experiments on the effect of heating and inhibitors on enzyme activity using laboratory experiments and chemical reaction equations with a minimum accuracy of 70%. | Biomolecules, Compound Reactions, Qualitative Analysis, Qualitative Analysis
ENZYMES: Effects of Heating and Inhibitors Against Enzyme Activity – Pancreatic amylase enzyme activity test – Pancreatic lipase enzyme activity test Students learn by doing tasks (protein experiments) that have been systematically designed. |
Project Based Learning | Students learn to show their performance and be responsible for their work to the assistant every week and present it in a discussion forum at the end of the practicum | FF: 1 x (3 x 60 min); ST:
1 x (3 x 60 min); SS: 1 x (3 x 60 min) |
(1). accuracy describes the principles and stages of protein analysis methods.
(2). The accuracy of applying the method in qualitative and quantitative analysis of protein-containing material samples. (3). Student activity in practicum and doing assignments (reports and presentations). |
10 |
| 5 | Able to describe (C2) the reaction principle and the stages of identification of organic and inorganic compounds and implement it (P2) in experiments on urine samples using laboratory experiments and chemical reaction equations with a minimum accuracy of 70%. | Biomolecules, Compound Reactions, Qualitative Analysis, Qualitative Analysis
URINE: Identification of Compounds in Urine Organic Compounds In Urine: – Breakdown of urea by urease – Test for reducing sugar – Test for creatinine A. JAFFE Experiment B. WEYL Experiment – Test for the presence of uric acid and its salts A. Muroxide Experiment B. Silver Reduction Experiment (SCHIFF) – Test for the presence of ketone compounds (experimental Rhotern) – Test for the presence of protein Inorganic Compounds In Urine: – Test for the presence of ammonia – Test for the presence of chloride – Test for the presence of phosphate and calcium |
Project Based Learning | Students learn by doing tasks (protein experiments) that have been systematically designed.
Students learn to show performance and account for their work to the assistant every week and present it in a discussion forum at the end of the practicum. |
FF: 1 x (3 x 60 min); ST:
1 x (3 x 60 min); SS: 1 x (3 x 60 min) |
(1). accuracy describes the principle of the reaction and the stages of identification of the presence of organic and inorganic compounds.
(2). The accuracy of implementing it (P2) in experiments on urine samples. (3). Student activity in practicum and doing assignments (reports and presentations). |
10 |
| 6 | Able to describe (C2) the principles and stages of basic microbiological techniques and implement them (P2) in making standard media and growing microorganisms in the laboratory with a minimum accuracy of 70%. | Biology, Biomolecules, Microbiology: Isolation and cultivation of microbes
– Manufacture of liquid nutrient medium – Preparation of nutrient agar medium – Bacterial isolation – Growing bacteria in liquid nutrient medium – Planting bacteria on agar slanted |
Project Based Learning | Students learn by doing tasks (protein experiments) that have been systematically designed.
Students learn to show their performance and be responsible for their work to the assistant every week and present it in a discussion forum at the end of the practicum |
FF: 1 x (3 x 60 min); ST:
1 x (3 x 60 min); SS: 1 x (3 x 60 min) |
(1). Accurately describes the principles and steps of basic microbiology techniques.
(2). The accuracy of implementing it in making standard media and growing microorganisms. (3). Student activity in practicum and doing assignments (reports and presentations). |
5 |
| 7 | Able to describe (C2) the principles and stages of basic microbiological techniques and implement them (P2) in making standard media and growing microorganisms in the laboratory with a minimum accuracy of 70%. | Biomolecules, Intermolecular Interactions
Isolation and Purification of the Early Stage of Alpha Enzymes Amylase |
Project Based Learning | Students learn by doing tasks (protein experiments) that have been systematically designed.
Students learn to show performance and account for their work to the assistant every week and present it in a discussion forum at the end of the practicum. |
FF: 1 x (3 x 60 min); ST:
1 x (3 x 60 min); SS: 1 x (3 x 60 min) |
(1). Accurately describe the principles and stages of basic techniques
of microbiology. (2). The accuracy of implementing it in making standard media and growing microorganisms. (3). Student activity in practicum and doing assignments (reports and presentations) |
10 |
| 8 | Able to describe (C2) the principle and technique of determining the specific activity of the enzyme and implement it (P2) in determining the specific activity of the -amylase enzyme in various samples with an accuracy of at least 70% | Biomolecules, Compound Reactions, Qualitative Analysis, Qualitative Analysis
Determination of Specific Activity of α-amylase Enzyme – Determination of Reducing Sugar Content by the Nelson-Somogyi Method – Determination of Protein Content by Lowry Method |
Project Based Learning | Students learn to show performance and account for their work to the assistant every week and present it in a discussion forum at the end of the practicum. | FF: 1 x (3 x 60 min); ST:
1 x (3 x 60 min); SS: 1 x (3 x 60 min) |
(1). accuracy describes the principles and techniques for determining the specific activity of enzymes
(2). The accuracy of implementing it in determining the specific activity of the -amylase enzyme in various samples. (3). Student activity in practicum and doing assignments (reports and presentations). |
5 |
| 9 | Able to describe (C2) the principles and techniques of DNA isolation and characterization and implement it (P2) in isolating and characterizing the DNA of various samples with a minimum accuracy of 70%. | Biomolecules, Intermolecular Interactions, Qualitative Analysis of DNA Isolation and Characterization
– DNA Isolation with a Simple Method – DNA characterization |
Project Based Learning | Students learn by doing tasks (lipid experiments) that have been systematically designed.
Students learn to show performance and account for their work to the assistant every week and present it in a discussion forum at the end of the practicum. |
FF: 1 x (3 x 60 min); ST:
1 x (3 x 60 min); SS: 1 x (3 x 60 min) |
(1). accuracy describes the principles and techniques of DNA isolation and characterization.
(2). The accuracy of applying it in isolating and characterizing the DNA of various samples. (3). Student activity in practicum and doing assignments (reports and presentations). |
5 |
| 10 | Able to describe (C2) the basic principles and techniques of computational phylogenetic analysis and implement it (P2) in determining the relationship between organisms based on their 16S rRNA gene sequence with a minimum accuracy of 70% | Computing, Bioinformatics: phylogenetic analysis
– Bioinformatics data retrieval via an internet database – Computational bioinformatics data preparation – Phylogenetic analysis using BIOEDIT and MEGA 6 software |
Project Based Learning | Students learn by doing tasks (lipid experiments) that have been systematically designed.
Students learn to show performance and account for their work to the assistant every week and present it in a discussion forum at the end of the practicum. |
FF: 1 x (3 x 60 min); ST:
1 x (3 x 60 min); SS: 1 x (3 x 60 min) |
(1). accuracy describes the basic principles and techniques of computational phylogenetic analysis.
(2). The accuracy of implementing it (P2) in determining the relationship between organisms based on the 16S rRNA gene sequence. (3). Student activity in practicum and doing assignments (reports and presentations). |
5 |
| 11 | Result Presentation | ||||||
| 12 | Response (Final Exam) | ||||||
Reference:
- Benyamin, H., (1960), Laboratory Manual of Biochemistry, fifth edition W.B. Sauders Company, Philadelphia, London
- Plummer T.D., (1978), An Introduction to Practical Biochemistry, Second edition, Tata McGraw-Hill Publishing Company Ltd, New Delhi.
- Capuccino, J. G. and Sherman, N., (2001), “Microbiological: A Laboratory Manual”, Addison-Wesley Publishing Company, New York, Page: 456.
- Scopes, R.K., (1998), “Protein Purification”, Springer-Verlag, New York, page: 45-54
- Colowick, S.P. and Kaplan, N.O. (1957) “Methods in Enzimology”, vol. III, Acad. Press. Inc., New York, 448-450
- Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11-15.
- Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013): MEGA6: Molecular evolutionary genetics analysis version 6.0, Mol. Biol. Evol., 30 (12), 2725–2729.
Glossary
GLO = Graduate Learning Outcome
CLO = Course Learning Outcomes
FF = Face to Face Learning
ST = Structured tasks
SS = Self Study
