Enzymology (ENZ)     

SEMESTER LEARNING PLAN

Document can be downloaded here

 

Course Title: Enzymology (ENZ)     

MK code: AKM21 450

Credit Weight: 2

Group of Courts: elective

Semester: 5

Prerequisite Course: Bio1

 

Lecturer:

Dra. Nies Suci Mulyani, MS

Purbowatiningrum Ria Sarjono., M.Si.

 

Graduate Learning Outcomes (GLO)

Attitude	GLO 1-(S9)	Demonstrate an attitude of responsibility for work in their field of expertise independently.
Knowledge	GLO 2-(PP1)	Mastering the theoretical concepts of structure, properties, changes, kinetics, and energetics of molecules and chemical systems, identification, separation, characterization, transformation, synthesis of macromolecular chemicals, and their application.
General Skills	GLO 3 -(KU1)	Able to apply logical, critical, systematic, and innovative thinking in the context of the development or implementation of science and technology that pays attention to and uses humanities values by their field of expertise
Special skill	GLO 4 -(KK2)	Able to solve science and technology problems in general and straightforward chemical fields such as identification, analysis, isolation, transformation, and synthesis of macromolecules through the application of knowledge of structure, properties, kinetics, and energetics of molecules and chemical systems, with analytical and synthesis methods in the field-specific chemistry, as well as the application of relevant technologies.
	GLO 5 -(KK2)	Able to analyze several alternative solutions in identification, analysis, isolation, transformation, and synthesis of available chemicals and present analysis conclusions for appropriate decision making.

 

 

Course Description

This course discusses the scope of enzymology, IUBMB-IUPAC enzyme classification, catalytic enzyme mechanism, enzyme kinetics and inhibition, enzyme activity testing, enzyme immobilization, and the application of enzyme technology.

Week	Expected ability (Sub-CLO)	Study Materials/ Learning Materials	Learning methods	Student Learning Experience	Time (minutes)	Evaluation
						Criteria and Indicators	%
1-2	Able to describe (C2) and implement (P2) the concept of enzymes, roles, and sources of enzymes from living cells and the relationship between enzyme and substrate interactions with a minimum accuracy of 70%.	Introduction: Scope of enzymology IUBMB-IUPAC classification of enzymes: Oxidoreductase(EC1), Transferase (EC 2.-), Hydrolase (EC 3.-), Lyase (EC 4.-), Isomerase (EC 5.-), Ligase (EC 6.-), Non-protein enzyme	Discovery learning Cooperative learning	Students learn by listening to lectures and actively discussing to explore further information to answer the problems asked by the lecturer.	FF: 1 x (2 x 50”) ST + SS: 1 x [(2 x 50”) + (2 x 60”)]	(1). Accurately describe the concept of the energy cycle and implement the idea of enzymes, the role, and source of enzymes from living cells, and the relationship between enzyme and substrate interactions (2). Student activity in discussions and doing assignments.	10
3-4	Students can describe (C2) and implement (P2) regarding the factors that support the catalysis mechanism of an enzyme with a minimum accuracy of 70%	Enzyme catalytic mechanism: Covalent catalysis, General acid-base catalysis, Metal ion catalysis, approximation catalysis	Discovery learning Cooperative learning Problem Based Learning	Before face-to-face class, students work on assignments: searching, collecting, and compiling information related to lecture study materials. During lectures, students listen to lectures and discuss the relationship between courses and their work.	FF: 1 x (2 x 50”) ST + SS: 1 x [(2 x 50”) + (2 x 60”)]	(1). Accurately describe the steps of ATP biosynthesis and implement the factors that support the mechanism of catalysis of an enzyme work (2). Student activity in discussions and doing assignments.	20
5-7	Students can describe (C2) the principles and mechanisms of Kinetics and Enzyme Inhibition (10.11,12,13) and implement them (P2) in processes in cells using schematic drawings and chemical reaction mechanisms with a minimum accuracy of 70%.	Kinetics and Enzyme Inhibition Steady-state kinetics Michaelis Menten, Lineweaver Burk plot, Hanes, Hofste plot, Catalytic efficiency, Two substrate reactions, Irreversible inhibition, Reversible inhibition (competitive, non-competitive, uncompetitive)	Discovery learning Cooperative learning Problem Based Learning	Before meeting face to face in class, students work on group assignments: prepare discussion materials in the form of papers (doc) and presentations (ppt). During lectures, students make presentations and discussions on the topic: reaction kinetics of enzyme inhibition and inhibition	FF: 1 x (2 x 50”) ST + SS: 1 x [(2 x 50”) + (2 x 60”)]	(1). Accurately describe the principles and mechanisms of kinetics and inhibition of enzymatic reactions (2). Student activity in discussions and doing assignments.	20
8		Midterm exam	Written exam		90	The truth and completeness of the answer to the question
9-10	Students can describe (C2) the principle of enzyme activity test and implement (P2) various methods for testing Enzyme activity (2) with a minimum accuracy of 70%.	Enzyme Activity Testing: Activity units, Direct vs. indirect testing, continuous vs. discontinuous, Spectrometric testing, Miscellaneous tests	Discovery learning Cooperative learning Problem Based Learning	Before face-to-face class, students work on assignments: searching, collecting, and compiling information related to lecture study materials. During lectures, students listen to lectures and discuss the relationship between courses and the work they are doing	FF: 1 x (2 x 50”) ST + SS: 1 x [(2 x 50”) + (2 x 60”)]	(1). Accuracy of describing Enzyme activity testing with several enzyme activity test methods (2). Student activity in discussions and doing assignments.	20
11-13	Students can describe (C2) the principle of Enzyme Immobilization (3 & 4) and implement it (P2) in the enzyme immobilization method with a minimum accuracy of 70%	Enzyme immobilization: Enzymatic reactor, Carrier binding method, Cross-linking method, Entrapping method	Discovery learning Cooperative learning Problem Based Learning	Before face-to-face class, students work on assignments: searching, collecting, and compiling information related to lecture study materials. During lectures, students listen to lectures and discuss the relationship between courses and their work.	FF: 1 x (2 x 50”) ST + SS: 1 x [(2 x 50”) + (2 x 60”)]	(1). Accuracy describes the enzyme immobilization method. (2). Student activity in discussions and doing assignments.	20
14-15	Students can describe (C2) the application of enzyme technology and apply it (P2) to enzyme technology in the pharmaceutical and health industries; in the food industry; chemical industry with a minimum accuracy of 70%	Enzyme technology application: Enzyme technology application in pharmaceutical and health industry, Enzyme technology application in the food industry, Enzyme technology application in the chemical industry	Discovery learning Cooperative learning Problem Based Learning	Before meeting face to face in class, students work on group assignments: prepare discussion materials in the form of papers (doc) and presentations (ppt). During lectures, students make presentations and discuss the topic 1 Application of enzyme technology in pharmaceutical and healthcare industries 2 Applications Applications of enzyme technology in the food industry	FF: 1 x (2 x 50”) ST + SS: 1 x [(2 x 50”) + (2 x 60”)]	(1). Accuracy in describing the application of enzyme technology in the industrial world (2). Student activity in discussions and doing assignments.	10
16	Final exams			Written exam	90	The truth and completeness of the answer to the question
Total Rating							100

 

Reference:

1. Fersht, A., 1998, Enzyme: Structure and Mechanism, WH Freeman & Co. N. York
2. Gul, S., Sredharan, SK., and Brocklehurst, K., 1998, Enzyme Assays, John Wiley and Sons
3. Kamp, RM., Choli-Papadopoulou, T., Wittmann-Liebold, B., 1997, Protein Structur Analysis, Springer-Verlag

Glossary

GLO = Graduate Learning Outcome

CLO = Course Learning Outcomes

FF = Face to Face Learning

ST = Structured tasks

SS = Self Study